Semester V

BTP 501 - Engineering and Environmental Botechnology
Total Hours Alloted : 60 Hours
Unit 1. Introduction to Genetic Engineering 1 Hour
Unit 2. Tools for genetic engineering
1. DNA manipulative enzymes Restriction enzymes and DNA ligases. 1 Hour
2. Gene cloning vectors Plasmids, BActeriophage and cosmids 3 Hours
Unit 3. In Vitro construction of recombinant DNA molecules (pBR 332, pUC 19) Isolation of
passenger & vector DNA, creation of r-DNA.
Unit-4. Transformation of r-DNA Transformation of r-DNA molecules into target host
organisms Calcium chloride mediated, Electorporation and microinjection. 2 Hours
Unit-5. Screening and selection of recombinant host cells Immunological screening and colony
hybridization.
Unit 6. Gene Libraries Genomic DNA and c DNA Cloning techiniques 2 Hours
Unit 7. Expression of cloned DNA in E.coli 1 Hours
Unit 8. Molecular biology techniques 6 Hours
a) Electrophoretic techniques Proteins and nucleic acids 6 Hours
b) Polyme ...more