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Q.

In the protocol for extraction of genomic DNA, 90% ethanol is used to precipitate DNA and 70% ethanol is used to wash the DNA pellet. Can anyone tell me why we use different concentrations and also the precise mechanism by which 90% and 70% solutions work?

Asked by malini rajan, 27 Feb '09 08:15 pm
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Answers (1)

1.

Alcohol is used to wash off some soluble pollutants, 90% is more concentrate extracts more of poll, than 70%
Answered by baymu, 27 Feb '09 08:16 pm

 
  
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